Menurut catatan resmi dari, mereka mengatakan bahwa YouTube sudah di akses oleh lebih dari 1 milliar pengguna di dunia, dan itu adalah angka pengguna yang fantastis. Karena pada aplikasi resmi dari YouTube, kita hanya bisa mendownload dalam format video saja, tidak ada pilihan untuk mendownloadnya sebagai lagu. Namun yang menjadi masalah di sini adalah ketika kita menemukan sebuah video di YouTube yang memiliki suara atau lagu yang unik, bagus, atau merdu, dan mempunyai keinginan untuk menyimpannya menjadi sebuah lagu tidak akan bisa. Hampir semua masyarakat di Indonesia sudah mengenal apa itu YouTube, terlebih lagi, YouTube dapat di gunakan oleh para content creator untuk mencari rezeki melalui video yang di unggahnya di YouTube. Cara mendownload lagu di komputer. Bagi kalian yang mengalami masalah serupa, namun ingin tetap menyimpannya sebagai lagu / audio atau dalam format.mp3, sebaiknya simak cara yang sudah kami rangkum di bawah ini beserta gambarnya. • 1.3k Downloads • Abstract Antagonistic Bacillus strains were isolated from soil and analyzed for the purpose of determining whether they could be used as natural biological agents. Primary in vitro screening for antagonism of the isolates was performed against five phytopathogenic mould fungi. Strains TS 01 and ZR 02 exhibited the most pronounced inhibitory effects. They were identified as Bacillus subtilis on the basis of their morphological, cultural and physiology-biochemical properties as well as their hierarchical cluster analysis conducted by means of computer program SPSS. The antimicrobial activity of the strains from cultural medium and sterile filtrate were determined in vitro against a great number of predominantly phytopathogenic fungi and bacteria. Bacillus subtilis is a remarkably diverse bacterial species – capable of growth within diverse environments including the gastrointestinal tracts of animals. Microarray-based comparative genomic analyses have revealed that members of this species also exhibit considerable genome diversity. Biochemical Test and Identification of Bacillus subtilis. Characteristics of Bacillus subtilis. Bacillus subtilis biochemical tests. TS 01 and ZR 02 strains exhibited very broad and at the same time degree varying antibiotic spectra of activities against both Gram-positive and Gram-negative microorganisms. Many of them were tested against sensitivity to the antimicrobial action of B. Subtilis for the very first time. Subtilis TS 01 and ZR 02 showed highest antifungal activity (sterile zone in diameter over 37 mm) against Alternaria solani, Botrytis cinerea, Monilia linhartiana 869, Phytophthora cryptogea 759/1 and Rhizoctonia sp. The most sensitive bacterial species were found to be Pseudomonas syringae pv. Tomato Ro and Xanthomonas campestris with sterile zones 48.0 and 50.0 mm in diameter, respectively. The latter draws a conclusion that the isolated and identified Bacillus subtilis strains are promising natural biocontrol agents and should be further studied and tested for control of numerous plant diseases. Wyss Institute for Biologically Inspired Engineering, Harvard University, USA DOI Published 2014-09-30 Accepted 2014-09-06 Received 2014-08-09 Academic Editor Subject Areas, Keywords Mechanotaxis, Elasticotaxis, Bacillus mycoides, Bacillus subtilis, Chain length, EDTA, Swarming Copyright © 2014 Polka and Silver Licence This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. Cite this article Polka JK, Silver PA. Induced sensitivity of Bacillus subtilis colony morphology to mechanical media compression. PeerJ 2: e597. Bacteria from several taxa, including Kurthia zopfii, Myxococcus xanthus, and Bacillus mycoides, have been reported to align growth of their colonies to small features on the surface of solid media, including anisotropies created by compression. While the function of this phenomenon is unclear, it may help organisms navigate on solid phases, such as soil. The origin of this behavior is also unknown: it may be biological (that is, dependent on components that sense the environment and regulate growth accordingly) or merely physical. Here we show that B. Subtilis, an organism that typically does not respond to media compression, can be induced to do so with two simple and synergistic perturbations: a mutation that maintains cells in the swarming (chained) state, and the addition of EDTA to the growth media, which further increases chain length. EDTA apparently increases chain length by inducing defects in cell separation, as the treatment has only marginal effects on the length of individual cells.
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